Editorial: Mouse eosinophils expressing Cre recombinase: endless “flox”ibilities
نویسندگان
چکیده
منابع مشابه
A Transgenic Mouse Line Expressing Cre Recombinase in Undifferentiated Postmitotic Mouse Retinal Bipolar Cell Precursors
Approaches for manipulating cell type-specific gene expression during development depend on the identification of novel genetic tools. Here, we report the generation of a transgenic mouse line that utilizes Vsx2 upstream sequences to direct Cre recombinase to developing retinal bipolar cells. In contrast to the endogenous Vsx2 expression pattern, transgene expression was not detected in prolife...
متن کاملMouse opsin promoter-directed Cre recombinase expression in transgenic mice.
PURPOSE Gene inactivation with homologous recombination in mice is a widely used tool to study gene function. However, many proteins play essential roles in a number of tissues and germline gene inactivation often results in embryonic lethality. To overcome this limitation and to dissect the functions of essential genes beyond embryonic development, we generated mouse rod opsin promoter-control...
متن کاملEfficient gene activation in mammalian cells by using recombinant adenovirus expressing site-specific Cre recombinase.
A recombinant adenovirus (Ad) expressing Cre recombinase derived from bacteriophage P1 was constructed. To assay the Cre activity in mammalian cells, another recombinant Ad bearing an on/off-switching reporter unit, where a LacZ-expression unit can be activated by the Cre-mediated excisional deletion of an interposed stuffer DNA, was also constructed. Co-infection experiments together with the ...
متن کاملTransgenic mice expressing Cre-recombinase specifically in M- or S-cone photoreceptors.
PURPOSE To establish lines of transgenic mice that express Cre-recombinase in M- or S-cone photoreceptors for generating cone photoreceptor-specific (conditional) mutants. METHODS Five kilobases of 5' upstream sequence of the mouse red-green (M) opsin gene or 0.5 kb of the mouse blue (S) opsin gene was cloned into a Cre-expression plasmid. Transgenic mice were generated and characterized, and...
متن کاملTransgenic mice expressing Cre-recombinase specifically in retinal rod bipolar neurons.
PURPOSE To establish a transgenic mouse line that expresses Cre-recombinase in retinal rod bipolar cells for the generation of rod bipolar cell-specific knockout mutants. METHODS The IRES-Cre-cDNA fragment was inserted into a 173-kb bacterial artificial chromosome (BAC) carrying the intact Pcp2 gene, by using red-mediated recombineering. Transgenic mice were generated with the modified BAC an...
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ژورنال
عنوان ژورنال: Journal of Leukocyte Biology
سال: 2013
ISSN: 0741-5400
DOI: 10.1189/jlb.0413191